Downstream Purification Scientist

Downstream Purification Scientist requires: Hands-on experience in Cesium chloride (CsCl) density gradient ultracentrifugation, ion-exchange chromatography, affinity purification, and tangential flow filtration (TFF), with a strong understanding of purification principles and data analysis. Masters degree in biology, biochemistry, chemical engineering, biotechnology, or related field. AAV or other viral vector CsCl density gradient and column purification. Experience with AKTA systems and Unicorn software. Downstream Purification Scientist duties: Execute purification processes including CsCl density gradient ultracentrifugation, affinity chromatography (e.g., AVB, CaptureSelect), ion-exchange chromatography (AEX, CEX), and other polishing steps. Operate AKTA systems (Avant, Pure, or Pilot) for small- to large-scale purification runs. Perform TFF operations for buffer exchange and concentration of intermediate or final products. Support experimental design, data collection, and documentation following established procedures and GMP/GLP best practices. Assist in troubleshooting and optimization of purification processes. Analyze in-process and purified samples using relevant analytical methods (e.g., UV, SDS-PAGE, ddPCR, ELISA, or HPLC). Collaborate cross-functionally with upstream, analytical, and manufacturing teams. Maintain lab equipment, manage buffers and column inventory, and ensure compliance with safety and documentation standards.